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目的:探求ALA口服在脑肿瘤光敏诊断中的最佳剂量、口服后最佳诊断时间、效果、阳性率、假阳性率、假阴性率及光谱分析的方法。方法:口服不同剂量的ALA(20mg/kg、40mg/kg、60mg/kg),不同时间(3小时、4小时、5小时、6小时)以激光诱导荧光OMA检测系统测定无脑瘤正常鼠二侧大脑半球,及G422脑胶质母细胞瘤模型的脑肿瘤、正常脑组织的荧光,并经各种方法进行数据处理,寻求脑肿瘤、正常脑组织相对值相差最大的合适的ALA口服剂量及诊断时间。诊断的阳性率、假阳性率、假阴性率。并将标本作病理检查。结果:脑肿瘤、正常脑组织相对值相差最小剂量及时间是在ALA40mg/kg,服ALA后4小时时比值最大。将标本作病理检查,诊断的阳性率为84%、假阳性率10%、假阴性率16%。结论:从实验数据分析口服ALA经OMA荧光光谱分析能作脑肿瘤荧光诊断,剂量以40mg/kg,服ALA后4小时左右作荧光光谱分析较合适。
OBJECTIVE: To explore the optimal dose of oral ALA for brain tumor photosensitivity, the best diagnosis time, effect, positive rate, false positive rate, false negative rate and spectral analysis after oral administration. METHODS: ALA (20mg / kg, 40mg / kg, 60mg / kg) was given orally at different doses for 3 hours, 4 hours, 5 hours and 6 hours, respectively. Laser- Lateral cerebral hemispheres and brain tumors of G422 brain glioblastoma model, normal brain tissue fluorescence, and data processing by various methods to find the appropriate ALA oral dose and maximum difference between normal brain tissue and brain tumors Diagnosis time. Diagnostic positive rate, false positive rate, false negative rate. And the specimens for pathological examination. Results: The minimum dose and time difference between the relative values of brain tumor and normal brain tissue were the highest in ALA40mg / kg and 4h after ALA. The specimens for pathological examination, the diagnosis of positive rate was 84%, false positive rate of 10%, false negative rate of 16%. CONCLUSION: Oral fluorescence of oral ALA can be used to diagnose brain tumors based on OMA fluorescence spectroscopy. The fluorescence spectra of 40 mg / kg and 4 hours after ALA administration are more suitable for fluorescence spectroscopy analysis.