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将来自甘蓝的BoFLC3基因和拟南芥的AtFT基因在芥菜中单独或共同表达发现,BoFLC3超量表达后,无论是长日照还是短日照条件下,转基因芥菜植株开花时间均明显延后;BoFLC3超量表达植株低温春化处理后,相对于未春化处理的转基因植株出现花期提前现象,但仍晚于非转基因对照植株;AtFT基因超量表达植株开花时间比非转基因对照植株大幅度提前。BoFLC3植株与AtFT植株杂交获得的BoFLC3和AtFT共表达植株开花时间与非转基因对照基本接近,显示BoFLC3和AtFT基因超表达对植物开花时间的影响效应可以相互抵消。同时,超量表达BoFLC3基因的芥菜植株抗寒性大幅度提高,而AtFT基因的超量表达不影响植株抗寒性,表明BoFLC3基因可能参与了植物的抗寒反应。
The BoFLC3 gene from Brassica napus and AtFT gene from Arabidopsis were expressed separately or co-expressed in Brassica juncea. After BoFLC3 overexpression, the flowering time of transgenic Brassica juncea plants was significantly delayed both in long-day and short-day conditions; Compared with non-transgenic control plants, flowering time was earlier than that of non-transgenic control plants. However, flowering time of AtFT gene overexpression plants was significantly earlier than non-transgenic control plants. The flowering time of BoFLC3 and AtFT co-expression plants obtained by crossing BoFLC3 plants with AtFT plants was basically close to that of non-transgenic control plants, indicating that the effect of BoFLC3 and AtFT gene overexpression on plant flowering time can be offset by each other. At the same time, the cold resistance of mustard plants overexpressing BoFLC3 gene increased significantly, while overexpression of AtFT gene did not affect plant cold resistance, indicating that BoFLC3 gene may be involved in the plant’s cold resistance.