论文部分内容阅读
目的 研究在不同环境中大出血死亡猪眼玻璃体液成分变化及其与死亡时间(postmorteminterval,PMI)的关系。方法 放血处死家猪后取192只猪眼随机分为A、B两组,分别在避光、温度(15±2)℃、湿度(50±5)%的空气中以及浸没于环境温度为(15±2)℃双蒸水的条件下放置2～96 h,采集玻璃体液,运用全自动生化检测仪、超高效液相色谱分析仪(Ultra Performance Liquid Chromatography,UPLC)检测K+、Na+、Cl–及次黄嘌呤(hypoxanthine,Hx)浓度;采用SPSS13.0统计软件对检测数据进行回归分析。结果 眼球置于空气中时,玻璃体液中K+、Hx与PMI相关性较高,回归方程为:PMI=-11.467+1.954[K+]-0.017[K+]2+0.511[Hx](R2=0.858);眼球置于双蒸水时,玻璃体液中Na+、Cl–与PMI相关性较高,回归方程为:PMI=144.439-1.636[Na+]+0.007[Na+]2-0.961[Cl-]+0.005[Cl-]2(R2=0.622)。结论 玻璃体液K+、Hx浓度变化可作为推断死后位于空气中的尸体较为稳定的指标;玻璃体液Na+、Cl–浓度变化可能对死后抛入淡水尸体的PMI推断具有参考价值。
Objective To study the changes of vitreous humor composition in pigs with bleeding due to major hemorrhage in different environments and their relationship with postmortemminterval (PMI). Methods A total of 192 pigs were randomly divided into A and B groups, respectively. The pigs were sacrificed in the air with darkness, temperature (15 ± 2) ℃, humidity (50 ± 5)% and immersed in the ambient temperature 15 ± 2 ℃ double distilled water for 2 ~ 96 h, the vitreous humor was collected and the contents of K +, Na + and Cl- were detected by the automatic biochemical analyzer and UPLC (UPLC) And hypoxanthine (Hx) were determined. The data were analyzed by SPSS13.0 software. Results When the eyeball was placed in the air, the correlation between K +, Hx and PMI in vitreous fluid was higher. The regression equation was: PMI = -11.467 + 1.954 [K +] - 0.017 [K +] 2 + 0.511 [Hx] ; When eyes were placed in double distilled water, the correlation between Na +, Cl- and PMI in vitreous fluid was higher. The regression equation was: PMI = 144.439-1.636 [Na +] + 0.007 [Na +] 2-0.961 [Cl-] + 0.005 [ Cl-] 2 (R2 = 0.622). Conclusions The changes of K + and Hx concentrations in vitreous humor may be used as a more stable index to estimate the post-mortem airborne corpses. The changes of Na + and Cl- concentrations in vitreous humor may be valuable for the PMI inference of freshwater dead bodies after death.