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采用聚丙烯酰胺凝胶电泳,对供试水稻不同品种9个部位6231个样本进行酯酶同工酶分析.重复性较好的酶带共有17条.不同部位酶带的条数各不相同.酶带和酶谱类型与品种形态特征有密切关系.同一个部位的酶带因品种而异,分为所有品种都出现的基本酶带及不是所有品种都会出现的鉴别酶带两种类型.以鉴别酶带条数为n,用M=2n可以估计该部位酶谱类型的数量M.各条酶带表达了不同等级的差异水平.各部位酶谱类型可以作为水稻品种鉴别和分类的一种生化指标.
Polyacrylamide gel electrophoresis was used to analyze the esterase isozymes of 6231 samples from 9 different rice cultivars. Repeatability of the enzyme with a total of 17. Different parts of the number of strips with different. Enzyme bands and zymogram types are closely related to the morphological characteristics of cultivars. The same part of the enzyme band varies by species, divided into all varieties are the basic enzyme bands and not all varieties will appear two types of identification enzyme band. To identify the number of enzyme bands n, with M = 2n can estimate the part of the number of zymotype M. Each band expressed different levels of difference. Zymogram types of each part can be used as a biochemical index for identification and classification of rice varieties.