论文部分内容阅读
【引言】由宫内营养不良导致的小于胎龄(SGA)状态与代谢综合征(MS)、2型糖尿病(T2DM)的发病关系密切。SGA成年后患MS、T2DM的发病机制复杂,其中胰岛功能障碍是联系SGA与成人代谢性疾病的一个重要环节。SGA者发生胰岛功能障碍的机制至今尚未完全阐明。钙信号是胰岛β细胞分泌胰岛素的一个关键调控因素,而高电压激活(HVA)的钙通道介导的Ca2+内流是导致β细胞内钙信号变化的主要原因。本研究利用新生大鼠胰岛β细胞分离培养技术和全细胞膜片钳技
【Introduction】 SGA due to intrauterine malnutrition is closely related to the development of metabolic syndrome (MS) and type 2 diabetes (T2DM). SGA adult suffering from MS, T2DM pathogenesis is complicated, in which islet dysfunction is an important link between SGA and adult metabolic diseases. The mechanism of pancreatic islet dysfunction in SGA has not yet been completely elucidated. Calcium signaling is a key regulator of insulin secretion by pancreatic beta cells, and calcium channel-mediated Ca 2+ influx by high-voltage activation (HVA) is the major cause of Ca 2+ signal changes in beta cells. In this study, newborn rat islet β-cell isolation and culture technology and whole-cell patch clamp technique