论文部分内容阅读
AIM:To investigate the activity of vesicular glutamate transporter-3(VGLUT3) in a visceral hyperalgesia rat model of irritable bowel syndrome,and the role of mast cells(MCs).METHODS:Transient intestinal infection was inducedby oral administration of Trichinella spiralis larvae in rats.On the 100th day post-infection(PI),the rats were divided into an acute cold restraint stress(ACRS)group and a non-stressed group.Age-matched untreated rats served as controls.The abdominal withdrawal reflex was used to measure the visceromotor response to colorectal distension(CRD).The expression levels of VGLUT3 in peripheral and central neurons were analyzed by immunofluorescence and western blotting.RESULTS:VGLUT3 expression in the L6S1 dorsal root ganglion cells was significantly higher in the PI group than in the control group(0.32±0.009 vs0.22±0.008,P<0.01),and there was no significant difference in the expression of VGLUT3 between MCdeficient rats and their normal wild-type littermates.Immunofluorescence showed that the expression levels of VGLUT3 in PI+ACRS rats were enhanced in the prefrontal cortex of the brain compared with the control group.CONCLUSION:VGLUT3 is involved in the pathogenesis of visceral hyperalgesia.Coexpression of c-fos,5-hydroxytryptamine and VGLUT3 after CRD was observed in associated neuronal pathways.Increased VGLUT3 induced by transient intestinal infection was found in peripheral nerves,and was independent of MCs.Moreover,the expression of VGLUT3 was enhanced in the prefrontal cortex in rats with induced infection and stress.
AIM: To investigate the activity of vesicular glutamate transporter-3 (VGLUT3) in a visceral hyperalgesia rat model of irritable bowel syndrome, and the role of mast cells (MCs). METHODS: Transient intestinal infection was induced by oral administration of Trichinella spiralis larvae in rats. On the 100th day post-infection (PI), the rats were divided into an acute cold restraint stress (ACRS) group and a non-stressed group. Age-matched untreated rats served as controls. The abdominal withdrawal reflex was used to The expression levels of VGLUT3 in peripheral and central neurons were analyzed by immunofluorescence and western blotting. RESULTS: VGLUT3 expression in the L6S1 dorsal root ganglion cells was significantly higher in the PI group than in the control group (0.32 ± 0.009 vs 0.22 ± 0.008, P <0.01), and there was no significant difference in the expression of VGLUT3 between MCdeficient rats and their normal wild-type littermates. Immunofluorescence orescence showed that the expression levels of VGLUT3 in PI + ACRS rats were enhanced in the prefrontal cortex of the brain compared with the control group. CONCLUSION: VGLUT3 is involved in the pathogenesis of visceral hyperalgesia. Cospression of c-fos, 5-hydroxytryptamine and VGLUT3 after CRD was observed in associated neuronal pathways. Increased VGLUT3 induced by transient intestinal infection was found in peripheral nerves, and was independent of MCs. More over, the expression of VGLUT3 was enhanced in the prefrontal cortex in rats with induced infection and stress.