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Cytosolic free calcium concentration plays a very important role in the regulation of platelet function Ganoderma Luchidum (GL) has antiplatelet aggregation The aim of this study is to evaluate the effect of GL on the platelet cytosolic free calcium concentration in rabbit in vivo The New Zealand rabbits were administered with GL at 2 0?g/kg two times a day for 2 weeks The measurement of Ca 2+ was performed using the calcium fluorescent dye Fura 2 at 510?nm emission with excitation wavelength of 340?nm before and after the administration of GL The resting Ca 2+ concentration ( ±s ) in platelet was 109 06±16 74?nmol/L (n=20) The addition of 0 5?μ/ml thrombin significantly increased the Ca 2+ concentration to 453 87±78 85?nmol/L (n=10, P <0 01) The increase rate of Ca 2+ was 331 40%±79 32% After administration of GL, the resting Ca 2+ concentration significantly decreased to 78 00±10 82?nmol/L The increase of Ca 2+ stimulating by 0 5?μ/ml thrombin was to 191 17±42 76?nmol/L ( P <0 01) The increase rate of Ca 2+ was 133 46%± 17 72% The resting Ca 2+ concentration before and after administration of GL was significantly different (n=20, P >0 01) and the increase rate before and after administration of GL also was significantly different (n=10, P <0 05)The results showed that GL can reduce the cytosolic free Ca 2+ concentration mediating by reducing Ca 2+ influx or increasing intracellular Ca 2+ store This may be an important mechanism of antiplatelet aggregation of GL
Cytosolic free calcium concentration plays a very important role in the regulation of platelet function Ganoderma Luchidum (GL) has antiplatelet aggregation The aim of this study is to evaluate the effect of GL on the platelet cytosolic free calcium concentration in rabbit in vivo The New Zealand rabbits were administered with GL at 2 0 μg / kg two times a day for 2 weeks The measurement of Ca 2+ was performed using the calcium fluorescent dye Fura 2 at 510 nm emission with excitation wavelength of 340 nm before and after the administration of GL The resting Ca 2+ concentration (± s) in platelet was 109 06 ± 16 74 nmol / L (n = 20) The addition of 0 5 μ / ml thrombin significantly increased the Ca 2+ concentration to 453 87 ± The increase rate of Ca 2+ was 331 40% ± 79 32% After administration of GL, the resting Ca 2+ concentration was significantly decreased to 78 00 ± 10 82 (n = 10, P <0.01) ? nmol / L The increase of Ca 2+ stimulating by 0 5? Μ / ml thrombin was to 191 17 ± 42 76? Nmol / L (P <0.01) The increase rate of Ca 2+ was 133 46% ± 17 72% The resting Ca 2+ concentration before and after administration of GL was significantly different (n = 20, P> 0 01) and the increase rate before and after administration of GL also was significantly different (n = 10, P <0 05) The results showed that GL can reduce the cytosolic free Ca 2+ concentration mediating by reducing Ca 2+ influx or increasing intracellular Ca 2+ store This may be an important mechanism of antiplatelet aggregation of GL