论文部分内容阅读
伤寒沙门氏菌Ｖｉ抗原系ａ─１，４─Ｎ─乙酰─半乳糖醛酸高度聚合体，其Ｃ２和Ｃ３位分别被Ｎ─乙酰基和０─乙酰基取代，它不能用分光光度法测定其含量，实验表明火箭电泳的火箭峰高与其样品中多糖含量的对数成直线相关关系，作者采用火箭电泳方法对全国六个生物制品研究所的１８批伤寒Ｖｉ多糖菌苗中Ｖｉ多糖含量进行了测定，结果表明多数制品符合规程要求，但部分制品多糖含量偏高，值得引起注意，各所三批制品差别不大，表明各所的生产工艺相对稳定，利用火箭电泳测定伤寒Ｖｉ多糖，操作简单，用时较短，所需样品极少，是目前较为合适的测定伤寒Ｖｉ多糖菌苗多糖含量的方法。
Salmonella typhi Vi antigens are a-1,4-N-acetyl-galacturonic acid highly polymeric, C2 and C3 positions were N-acetyl and 0 ─ acetyl substitution, it can not be determined by spectrophotometry The experiments show that rocket electrophoresis rocket peak height and its sample polysaccharide content of the logarithm of a linear relationship between the authors using rocket electrophoresis of six National Institute of Biological Vi probe polysaccharide eighteen batches of vaccine were determined , The results showed that most products meet the regulatory requirements, but some products polysaccharide content is high, it is worth attention, the three batches of products are not very different, indicating that the production process is relatively stable, the rocket electrophoresis of typhoid Vi polysaccharide, simple operation, Short, very few samples required, is currently more appropriate method for the determination of tyrosinase Vi polysaccharide polysaccharide content.