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在水稻自花授粉 1～ 3h后 ,利用其形成的花粉管通道 ,将提取的含有抗白叶枯病Xa2 1基因的水稻IRBB2 1的总DNA直接导入受体水稻豫粳 6号中 ,经白叶枯病菌接种鉴定 ,在D1代获得抗白叶枯病材料 3株。利用RAPD技术对该组合的受体、供体及转化后代进行分析 ,证明供体DNA片段已整合到受体基因中。
After self-pollination of rice for 1-3 h, the total DNA of the extracted IRBB2 1 containing rice bacterial blight resistance Xa2 1 gene was directly introduced into the recipient rice Yujing 6 using the pollen tube channel formed by it, Inoculation identification of leaf blight, obtained in the D1 generation of three materials resistant to bacterial leaf blight. The combined receptor, donor and progeny were analyzed using RAPD technology to demonstrate that the donor DNA fragment had been integrated into the recipient gene.