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目的 探讨氧化修饰低密度脂蛋白 (OX- LDL)对血管内皮细胞损伤的机制。方法 在人脐静脉内皮细胞体外培养的基础上 ,通过采用 3H-胸腺嘧啶核苷 (3H- Td R)掺入 DNA技术和硫代巴比妥酸比色法 ,分别分析了 OX- LDL对内皮细胞 DNA合成及脂质过氧化物 (LPO)的代谢产物丙二醛 (MDA)含量的影响。结果 当内皮细胞暴露于 OX- LDL(50～ 2 0 0μg/ ml) 2 4 h后 ,其 3H- Td R掺入明显减少 ,但 MDA含量显著增加。结论 OX- LDL能够明显抑制内皮细胞 DNA合成 ,并显著增加内皮细胞的脂质过氧化物含量。
Objective To investigate the mechanism of oxidatively modified low density lipoprotein (OX-LDL) on vascular endothelial cell injury. Methods Human umbilical vein endothelial cells were cultured in vitro, and the effects of OX-LDL on endothelium were analyzed by 3H-thymidine incorporation (3H-TdR) DNA and thiobarbituric acid colorimetric methods respectively. Cell DNA synthesis and lipid peroxidation (LPO) metabolites malondialdehyde (MDA) content. Results When endothelial cells were exposed to OX-LDL (50 ~ 200 μg / ml) for 24 h, 3H-Td R incorporation was significantly reduced but MDA content was significantly increased. Conclusion OX-LDL can significantly inhibit endothelial cell DNA synthesis and significantly increase the lipid peroxidation of endothelial cells.