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目的探讨凋亡相关基因bax和bcl-xl蛋白表达水平与非小细胞肺癌(NSCLC)临床病理特征的关系。方法应用免疫组化SP法检测了35例NSCLC和7例正常肺组织中bax和bcl-xl蛋白的表达。结果bax在正常肺组织中表达率为71.4%,在NSCLC中表达率为42.9%,提示bax蛋白在NSCLC中表达过低。bcl-xl在正常肺组织中表达率为14.3%,在NSCLC中的表达率为57.1%,提示NSCLC中存在bcl-xl蛋白高表达。bax与TNM分期(r=-0.475,P=0.002)、淋巴结转移(r=-0.236,P=0.015)负相关,bcl-xl与TNM分期(r=0.542,P=0.027)、淋巴结转移(r=0.257,P=0.003)、病理分级(r=0.183,P=0.024)正相关。bax和bcl-xl间负相关(r=-0.162,P=0.032),其他组间未见显著相关性。结论bax和bcl-xl蛋白相互抑制,均与NSCLC的发生、发展有关,可作为反映NSCLC生物学行为的指标。
Objective To investigate the relationship between the expression of bax and bcl-xl protein and the clinicopathological features of non-small cell lung cancer (NSCLC). Methods The expressions of bax and bcl-xl in 35 NSCLC and 7 normal lung tissues were detected by immunohistochemical SP method. Results The expression of bax was 71.4% in normal lung tissue and 42.9% in NSCLC, suggesting that bax protein was overexpressed in NSCLC. The expression rate of bcl-xl in normal lung tissue was 14.3% and in NSCLC was 57.1%, which indicated that bcl-xl protein was highly expressed in NSCLC. bax was negatively correlated with TNM stage (r = -0.475, P = 0.002) and lymph node metastasis (r = -0.236, P = 0.015) = 0.257, P = 0.003), pathological grade (r = 0.183, P = 0.024). There was a negative correlation between bax and bcl-xl (r = -0.162, P = 0.032). No significant correlation was found between other groups. Conclusions The mutual inhibition of bax and bcl-xl proteins is related to the occurrence and development of NSCLC and may be used as an index to reflect the biological behavior of NSCLC.