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目的:应用浓氨水致小鼠咳嗽模型,筛选出枇杷花提取物镇咳作用最优的有效剂量。方法:将经筛选后的小鼠随机分为30个组,即模型对照组、阳性药对照组、枇杷叶浸膏15个剂量组和枇杷花提取物13个剂量组。连续灌胃给药3天后观察药物对浓氨水致小鼠咳嗽的影响,观察指标为三分钟内咳嗽次数(次)和咳嗽潜伏期(s)。结果:枇杷花提取物在140.59~25000mg(生药)/kg和枇杷叶浸膏在150~47434.16mg(生药)/kg的剂量范围内均能非常明显地减少小鼠三分钟内咳嗽次数,但二者的各剂量对咳嗽潜伏期的影响都不明显。对枇杷花提取物在70.09~25000mg(生药)/kg的剂量范围内的镇咳作用进行重复验证,结果亦显示250mg(生药)/kg剂量的镇咳作用最佳。结论:从有效性和制剂可行性方面综合考虑,最终确定枇杷花提取物相对合理的镇咳剂量为250 mg(生药)/kg,折算成成人日用剂量为25mg(生药)/kg。
Objective: To study the cough model induced by concentrated ammonia in mice, and to screen out the most effective and effective dose for the antitussive effect of the extract of loquat flower. Methods: The screened mice were randomly divided into 30 groups: model control group, positive control group, 15 doses of loquat leaf extract and 13 doses of loquat flower extract. Three days after continuous gavage, the effect of the drug on cough caused by concentrated ammonia water in mice was observed. The observation indexes were cough times (times) and cough latency (s) in three minutes. Results: The results showed that the extract of loquat in 140.59 ~ 25000mg (crude drug) / kg and loquat leaf extract in the dose range of 150 ~ 47434.16mg (crude drug) / kg can significantly reduce the number of cough within three minutes in mice, but two The effects of various doses on cough latency were not significant. The antitussive effect of the extract of loquat flower in the dose range of 70.09-25000mg / kg was repeated and the result also showed that the antitussive effect of 250mg / kg dose was the best. Conclusion: Considering the availability and feasibility of the preparation, the relatively reasonable antitussive dose of the extract of loquat flower is 250 mg (crude drug) / kg, which is converted into an adult daily dose of 25 mg (crude drug) / kg.