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AIM:To investigate the expression of three types of mucin(MUC1,MUC2,MUC5AC)and E-cadherin in human gastriccarcinomas and their clinical significance.METHODS:Ninety-four gastric cancer specimens wereclassified according to WHO criteria and detected by immun-ohistochemical assay of expression of mucins and E-cadherin.RESULTS:The positive expression rates of MUC1,MUC2,MUC5AC and E-cadherin were 82%(77/94),84%(79/94),40%(38/94)and 56%(53/94)respectively.MUC1 expressionwas significantly correlated with the types of cancer(thepositive rates of MUC1 in well and moderately differentiatedtubular adenocarcinoma,poorly differentiated adenocardnoma,signet-ring cell carcinoma and mucinous carcinoma were91%,87%,71%,71%,respectively,P<0.05),age ofpatients(the positive rates of it among the people who areyounger than 40 years,between 40-60 years and over 60 yearwere 74%,81%,89%,P<0.05),lymph nodes involvement(the positive rates in the non-interfered group and the interferedgroup were 78%,85%,P<0.05)and tumor size(the positiverates in the tumors with the size less than 3 cm,3-6 cmand larger than 6 cm were 69%,92%,69%,P<0.05); MUC2expression was significantly associated with types of cancersand had the strongest expression in mucinous carcinomas(the positive rates of MUC2 in well and moderately differentiatedtubular adenocarcinorna,poorly differentiated adenocardnoma,signet-ring cell carcinoma and mucinous carcinoma were94%,70%,81%,100%,P<0.05),but it had no obvious relationto age,gender,tumor location,lymph nodes involvement,depth of invasion and metastasis to extra-gastric organs(P>0.05);MUC5AC expression was not related to any ofthe characteristics investigated except that it had relationto gender,whereas MUC5AC showed the tendency to higherexpression in less invasive lesions and lower expression inadvanced stage cancers(P>0.05);No significant differencewas found for E-cadherin expression.There were strongpositive relationships between the expression of MUC1and E-cadherin,MUC2 and E-cadherin,MUC1 and MUC2(R=0.33,R=0.22,R=0.32,respectively,P<0.05).Accordingto the COX proportional hazards model,older patients, involvement of lymph nodes,different types of gastriccancer and MUC2 expression were significantly associatedwith poorer outcome of gastric cardnoma patients(β=0.08,β=3.94,β=1.33,β=0.75,respectively,P<0.05).CONCLUSION:MUC1 and MUC2 are good markers of differenttypes of gastric cancer.MUC2 is especially a good markerof mucinous carcinoma.MUC1,MUC2 may interfere withthe function of E-cadherin in gastric carcinomas,and havesynergic effect on progression of gastric cancers.
AIM: To investigate the expression of three types of mucin (MUC1, MUC2, MUC5AC) and E-cadherin in human gastric carcinomas and their clinical significance. METHODS: Ninety-four gastric cancer specimens were classified according to WHO criteria and detected by immuno- ohistochemical assay of expression of mucins and E-cadherin. RESULTS: The positive expression rates of MUC1, MUC2, MUC5AC and E-cadherin were 82% (77/94), 84% (79/94), 40% MUC1 expression was significantly correlated with the types of cancer (the positive rates of MUC1 in and moderately differentiated tubular adenocarcinoma, poorly differentiated adenocardium, signet-ring cell carcinoma and mucinous carcinoma were 91%, 87%, 71% , 71%, respectively, P <0.05), age of patients (the positive rates of it among the people who are younger than 40 years, between 40-60 years and over 60 yearwere 74%, 81%, 89%, P <0.05) , the lymph nodes involvement (the positive rates in the non-interfered group and the interfered group were 78%, 85%, P <0.05) an d tumor size (the positiverates in the tumors with the size less than 3 cm, 3-6 cmand larger than 6 cm were 69%, 92%, 69%, P <0.05); MUC2expression was significantly associated with types of cancersand had the strongest expression in mucinous carcinomas (the positive rates of MUC2 in and moderately differentiated tubular adenocarcinorna, poorly differentiated adenocardium, signet-ring cell carcinoma and mucinous carcinoma were 94%, 70%, 81%, 100%, P <0.05) no obvious relationto age, gender, tumor location, lymph nodes involvement, depth of invasion and metastasis to extra-gastric organs (P> 0.05); MUC5AC expression was not related to any of these characteristics noted except that it had had to gender, MUC5AC showed the tendency to higherexpression in less invasive lesions and lower expression in advanced stage cancers (P> 0.05); No significant difference was found for E-cadherin expression. There were strong positive relationships between the expression of MUC1 and E-cadherin, MUC2 anAccording to the COX proportional hazards model, older patients, involvement of lymph nodes, different types of gastriccancer and MUC2 expression (R = 0.33, R = 0.22, R = 0.32, respectively, were significantly associated with poorer outcome of gastric cardnoma patients (β = 0.08, β = 3.94, β = 1.33, β = 0.75, respectively, P <0.05) .CONCLUSION: MUC1 and MUC2 are good markers of different types of gastric cancer. a good marker of mucinous carcinoma. MUC1, MUC2 may interfere with the function of E-cadherin in gastric carcinomas, and have synergic effect on progression of gastric cancers.