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目的：探讨中药复方肝欣液体外抗乙肝病毒（HBV）活性及抑制乙肝表面抗原（HBsAg）的作用。方法：采用2．2．15细胞培养测定肝欣液对HBV的活性以及采用反相被动血凝抑制试验（RPHI）对肝欣液抗HBsAg进行实验观察。结果：肝欣液最高浓度为5～7μl／ml，作用7天和14天时，对2．2．15细胞HBsAg分泌抑制率分别为55.12％～57．48％和60．98％～71．34％，并可使培养上清中HBVDNA的量减少；浓度为7μl／ml，作用14天时对乙肝e抗原（HBeAg）分泌抑制率为50．28％，并可使培养上清HBVDNA的量减少，肝欣液500μl／ml对HBsAg有显著抑制作用，与阴性对照组比较凝集效价下降3～4个倍比度（P＜0.01）。结论：肝欣液浓度为5～7μl／ml时，在体外细胞培养中，具有一定的抗HBV活性，并对HBsAg有良好的抑制作用。
Objective: To investigate the anti-hepatitis B virus (HBV) activity and the inhibitory effect of hepatitis B surface antigen (HBsAg) in vitro. Methods: 2.2.15 cell culture was used to determine the activity of Gansu liquid on HBV and the anti-HBsAg activity of Ganshen liquid was observed by reverse phase hemagglutination inhibition test (RPHI). Results: The maximum inhibitory rates of HB-Ag in 2.2.15 cells were 55.12% -57.48% and 60.98% -71.34 %, And can reduce the amount of HBVDNA in culture supernatant; the concentration of 7μl / ml, the effect of 14 days on the secretion of hepatitis B e antigen (HBeAg) inhibition was 50.28%, and the amount of HBVDNA can reduce the culture supernatant, Hepatexin 500μl / ml had a significant inhibitory effect on HBsAg, and the agglutination titer decreased by 3 ~ 4 times (P <0.01) compared with the negative control group. Conclusion: When the concentration of hepatic extract is 5 ~ 7μl / ml, it has certain anti-HBV activity in in vitro cell culture and has a good inhibitory effect on HBsAg.