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采用盆栽试验,研究砷污染土壤中施用黄腐酸(FA)对烤烟氮代谢及其过程的影响。结果表明,与砷污染土壤相比,施用黄腐酸提高了烤烟的硝酸还原酶(NR)活性,降低了谷氨酰胺合成酶(GS)活性,改善了NO3--N的同化能力,抑制了NH4+-N的同化。不同FA施用量对氮转化代谢的影响不同,在团棵期,虽然黄腐酸的施用提高了谷氨酸-丙酮酸转氨酶(GPT)活性,但随着生育进程的推进,适量的黄腐酸最终能抑制氮转化代谢的旺盛问题,使谷氨酸-丙酮酸转氨酶(GPT)、天冬酰胺酶(ASG)活性和游离氨基酸含量降低。砷污染土壤中施用黄腐酸还可降低烤烟团棵期的蛋白质含量,提高现蕾以后的蛋白质含量和全生育期的烟碱含量。本试验条件下,以土壤中施用600mg/kg的FA对减轻砷毒害效果最好。
Pot experiments were conducted to study the effects of FA on the nitrogen metabolism and the process of flue-cured tobacco in arsenic-contaminated soils. The results showed that compared with arsenic - contaminated soil, fulvic acid increased nitrate reductase (NR) activity, reduced glutamine synthetase (GS) activity, improved NO3 - N assimilation ability and inhibited Assimilation of NH4 + -N. The effect of different FA application rates on nitrogen metabolism was different. During the boll stage, although the application of fulvic acid increased glutamic acid-pyruvate transaminase (GPT) activity, with the progress of fertility, the appropriate amount of fulvic acid Eventually, it inhibits the exuberant nitrogen metabolism and decreases the activity of glutamate pyruvate aminotransferase (GPT), asparaginase (ASG) and free amino acids. The application of fulvic acid in arsenic-contaminated soil can also reduce the protein content in the roasted stage and increase the protein content after budding and the nicotine content in the whole growth period. Under the experimental conditions, the application of 600 mg / kg FA in soil has the best effect on reducing arsenic toxicity.