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为探讨丙型肝炎病毒（ＨＣＶ）与ＨＣＶ感染者血清丙氨酸转氨酶（ＡＬＴ）的关系，本文应用ＨＣＶＮＳ５区逆转录－套式聚合酶链反应（ＲＴ－ＰＣＲ）对７９例抗－ＨＣＶ阳性者进行ＨＣＶＲＮＡ检测，同时用限制性内功酶法对ＨＣＶ进行基因分型。检出ＨＣＶＲＮＡ阳性者６９例，其中ＨＣＶⅡ型３０例，Ⅲ型２７例，Ⅱ／Ⅲ混合型１２例，ＨＣＶ不同的基因型在ＡＬＴ正常组和异常组中的分布有显著性差异（Ｐ＜０．０１），Ⅱ型和Ⅱ／Ⅲ混合型主要分布于ＡＬＴ异常组中，Ⅲ型主要分布于ＡＬＴ正常组中。提示Ⅱ型和Ⅱ／Ⅲ混合型很可能是导致ＡＬＴ异常的主要型别，应引起足够重视。
In order to explore the relationship between hepatitis C virus (HCV) and serum alanine aminotransferase (ALT) in HCV-infected patients, 79 anti-HCV positives were detected by RT-PCR using HCVNS5 reverse transcriptase-polymerase chain reaction The HCV RNA was detected and HCV genotyped by restriction endonuclease method. There were 69 cases with positive HCVRNA, including 30 cases of HCV Ⅱ, 27 cases of type Ⅲ and 12 cases of mixed type Ⅱ / Ⅲ. The distribution of HCV genotypes in normal and abnormal ALT groups was significantly different (P <0 .01). Type Ⅱ and Ⅱ / Ⅲ mixed type were mainly distributed in ALT abnormal group, and type Ⅲ mainly distributed in normal ALT group. Prompt type Ⅱ and Ⅱ / Ⅲ mixed type is likely to lead to the main type of abnormal ALT, should cause enough attention.