论文部分内容阅读
目的研究婴幼儿增生期血管瘤组织中Livin和Smac的表达并进行相关性分析,初步探讨Livin和Smac在血管瘤发病机制中的作用。方法采用RT-PCR和免疫组化分别检测LivinmRNA、SmacmRNA和蛋白在20例婴幼儿增生期血管瘤和15例正常皮肤组织中的表达。结果以上2种组织中LivinmRNA、SmacmRNA和蛋白的表达,其差异均有统计学意义(P<0.05)。血管瘤中LivinmRNA的表达水平和蛋白的阳性表达率高于正常皮肤组织(P<0.05),血管瘤中SmacmRNA的表达水平和蛋白的阳性表达率低于正常皮肤组织(P<0.05);增生期血管瘤组织中Livin和Smac蛋白的表达呈负关联。结论Livin基因高表达可能抑制了Smac基因表达,从而导致了血管瘤内皮细胞增殖和凋亡的失衡,最终导致了血管瘤的形成。
Objective To study the expression of Livin and Smac in infantile hyperplastic hemangiomas and to analyze the relationship between Livin and Smac in the pathogenesis of hemangiomas. Methods RT-PCR and immunohistochemistry were used to detect the expression of Livin mRNA, SmacmRNA and protein in 20 infantile hyperplastic hemangiomas and 15 normal skin tissues respectively. Results The expressions of Livin mRNA, Smac mRNA and protein in the above two tissues were significantly different (P <0.05). The expression of Livin mRNA and protein in hemangiomas were higher than those in normal skin tissues (P <0.05). The expression of SmacmRNA and protein in hemangiomas were lower than those in normal skin tissues (P <0.05) The expression of Livin and Smac in hemangiomas was negatively correlated. Conclusion The high expression of Livin gene may inhibit the expression of Smac gene, leading to the imbalance of proliferation and apoptosis of endothelial cells in hemangiomas, which ultimately leads to the formation of hemangiomas.