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[目的]探讨双糖链蛋白聚糖Biglycan对大肠癌细胞HT-29和SW480增殖抑制作用及其机制。[方法]采用BrdU法和细胞克隆形成实验检测Biglycan对大肠癌细胞HT-29和SW480的增殖能力影响;Hoechst染色检测细胞凋亡情况;WesternBlot法检测加药处理前后细胞内细胞周期相关蛋白CyclinD1、CyclinA、p21和p27表达变化。[结果]Biglycan呈剂量依赖性的抑制大肠癌细胞HT-29和SW480生长,100nmol/LBiglycan和50nmol/LBiglycan浓度作用即可诱导HT-29、SW480细胞凋亡。经Biglycan处理后,细胞内CyclinD1和CyclinA表达出现下调趋势,p21和p27表达有所增加。[结论]Biglycan可能抑制大肠癌细胞HT-29和SW480增殖,诱导细胞凋亡、下调CyclinD1和CyclinA表达和上调p21和p27表达。
[Objective] To investigate the inhibitory effect of Biglycan, a big glycansan, on the proliferation of colorectal cancer cells HT-29 and SW480 and its mechanism. [Methods] The effects of Biglycan on the proliferation of colorectal cancer cells HT-29 and SW480 were detected by BrdU assay and cell clone formation assay. The apoptosis of colorectal cancer cells HT-29 and SW480 was detected by Hoechst staining. The expressions of CyclinD1, CyclinA, p21 and p27 expression changes. [Result] Biglycan inhibited the growth of HT-29 and SW480 cells in a dose-dependent manner. The apoptosis of HT-29 and SW480 cells was induced by 100nmol / LBiglycan and 50nmol / LBiglycan. After Biglycan treatment, the expression of CyclinD1 and CyclinA in cells decreased, and the expression of p21 and p27 increased. [Conclusion] Biglycan may inhibit the proliferation of HT-29 and SW480 cells, induce apoptosis, down-regulate the expression of CyclinD1 and CyclinA, and up-regulate the expression of p21 and p27.