非水介质毛细管电泳电导检测虎杖中的白藜芦醇

• 作 者: 郑妍鹏 李晓东 莫金垣 谢天尧 李凤屏 李娜
• 机 构: 中山大学化学与化学工程学院,中山大学化学与化学工程学院,中山大学化学与化学工程学院,中山大学化学与化学工程学院,中山大学化学与化学工程学院,中山大学化学与化学工程学院 广州510275,广州51027
• 出 处: 应用化学
• 发表日期: 2002年06期
• 关键词: 白藜芦醇 虎杖 分离检测 非水介质毛细管电泳

Capillary zone electrophoresis(CZE) has been performed for determination of resveratrol in Rhizoma Polygoni Cuspidati in a non aqueous solution of tris(hydroxymethyl)aminomethane(THAM) and boric acid with methanol as solvent. The effects of solvent, electrolyte, pH, concentration, running time and voltage were examined. The total separation and determination could be completed within 11 min. Under the optimum conditions, the linear range of trans resveratrol was 27~ 540 mg/L . The RSD( n =6) of peak area for resveratrol was 2 46% and the recovery was 103 4%.

The effects of solvent, electrolyte, pH, concentration, pH, and concentration on resveratrol in Rhizoma Polygoni Cuspidati in a non aqueous solution of tris (hydroxymethyl) aminomethane (THAM) and boric acid The total separation and determination could be completed within 11 min. Under the optimum conditions, the linear range of trans resveratrol was 27-540 mg / L. The RSD (n = 6) of peak area for resveratrol was 2 46% and the recovery was 103 4%.