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应用SYFPEITHI等七种软件对人、鼠源血小板糖蛋白GPⅡb/Ⅲa抗体蛋白进行HLA-A*0201、HLA-A*1101和HLA-A*2401限制性表位预测,经去除已发表多肽,取前者覆盖后两者多肽为混合CTL表位;再联合predTAP等TAP结合预测软件结果及NetChop等蛋白酶切位点预测软件结果,取包含这两者的多肽为CTL修正表位。用基于肽MHC-Ⅱ结合算法Syfpeithi等软件进行HLA-DR Th表位预测,最后取Th表位覆盖CTL修正表位而得T细胞混合表位。用DNAstar软件子程序Protean软件对两条蛋白链进行二级结构、B细胞表位预测。从1 740条多肽中筛选出人源GPⅡb/Ⅲa抗体5条T细胞混合表位肽:1-15、24-38、50-64、65-81、109-121,7条B细胞表位肽:5-9、22-30、40-46、55-71、80-90、100-105、110-115;鼠源GPⅡb/Ⅲa抗体5条T细胞混合表位肽:1-15、26-40、46-60、68-82、93-107,5条B细胞表位肽:5-10、38-43、58-70、77-84、99-105。
HLA-A * 0201, HLA-A * 1101 and HLA-A * 2401 restricted epitopes were predicted by using seven kinds of software SYFPEITHI and others. The former covers the latter two peptides as mixed CTL epitopes; combined with predTAP and other TAP binding prediction software results and NetChop protein cleavage site prediction software results, take both peptides as CTL modified epitopes. HLA-DR Th epitope was predicted by software such as Syfpeithi based on peptide MHC-Ⅱ binding algorithm. Finally, Th epitope was used to cover CTL to modify the epitope to obtain T cell mixed epitope. Using DNAstar software program Protean software secondary structure of two protein chains, B cell epitopes prediction. From 1 740 polypeptides, five T cell mixed epitope peptides of human GP IIb / IIIa antibody were screened: 1-15, 24-38, 50-64, 65-81, 109-121 and 7 B-cell epitope peptides : 5-9, 22-30, 40-46, 55-71, 80-90, 100-105, 110-115; mouse GP IIb / IIIa antibody 5 T cell mixed epitope peptide: 1-15,26- 40, 46-60, 68-82, 93-107, 5 B-cell epitope peptides: 5-10, 38-43, 58-70, 77-84, 99-105.