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乙型肝炎病毒(HBV)不能在非灵长类动物体内增殖,组织培养分离病毒亦未成功,加之该病毒结构成份复杂,又常掺杂有宿主蛋白,因此各种诊断方法需要依赖高度敏感的免疫血清学试验和高度特异性的抗体.淋巴细胞杂交瘤技术能产生大量质量均一的具有高度特异性的单克隆抗体(McAb).目前,已经制成了针对HBV在血清学上可以识别的几种抗原——表面抗原(HBsAg)、e抗原(HBeAg)和核心抗原(HBcAg)的McAb.本文仅就近年来国外在抗HBV各种抗原的McAb的研制和应用方面作一综述.
Hepatitis B virus (HBV) can not proliferate in non-primate animals. Tissue culture and virus isolation has not been successful. In addition, the virus is complicated in structure and often doped with host proteins. Therefore, various diagnostic methods need to be highly sensitive Immune serological tests and highly specific antibodies.Lymphocyte hybridoma technology can produce a large number of homogeneous mass with high specificity of monoclonal antibodies (McAb) .Has been made for HBV serologically identifiable Antigen - surface antigen (HBsAg), e antigen (HBeAg) and the core antigen (HBcAg) McAb.In this paper, only in recent years in the anti-HBV anti-HBV McAb development and application are reviewed.