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目的 观察宇宙辐射对民航飞行人员淋巴细胞微核的影响。 方法 采集 6 5名健康飞行人员和 2 1名地面对照人员的外周静脉血 ,在含 PHA的 RPMI16 40培养基中 37℃培养 72 h,经低渗、固定、涂片、染色 ,每例观察 10 0 0个转化淋巴细胞中的微核。 结果 飞行人员的 MNF(5 .5 1±0 .2 9‰ )、MNCF(4 .92± 0 .2 7‰ )与地面人员的 MNF(3.0 5± 0 .38‰ )、MNCF(2 .6 2± 0 .35‰ )相比 ,差异有非常显著性意义 (P<0 .0 1)。MNF、MNCF随飞行人员的年飞行小时、飞行工龄及飞行状况变化而变化的梯度小 ,差异无显著性意义 (P>0 .0 5 )。在性别和年龄可比条件下 ,吸烟飞行人员 MNF(6 .12± 0 .42‰ )、MNCF(5 .5 0± 0 .40‰ )略高于不吸烟飞行人员 MNF(5 .19± 0 .5 0‰ )、MNCF(4 .48± 0 .46‰ ) ,但差异无显著性意义 (P>0 .0 5 )。 结论 宇宙辐射引起民航飞行人员染色体损伤。常规培养微核法灵敏度低 ,不能反映飞行负荷的差别。吸烟没有明显加重飞行人员染色体损伤
Objective To observe the effects of cosmic radiation on the micronuclei of lymphocytes of civil aviation pilots. Methods Peripheral venous blood was collected from 65 healthy pilots and 21 ground controls and cultured in RPMI1640 medium containing PHA at 37 ℃ for 72 h. The cells were observed for hypotension, fixation, smear and staining for 10 cases Micronuclei in 0 0 transformed lymphocytes. Results The MNF (5.51 ± 0.29%), MNCF (4.92 ± 0.27%) and ground staff MNF (3.05 ± 0.38%), MNCF (2.6 2 ± 0. 35 ‰), the difference was significant (P <0.01). MNF, MNCF with the pilot’s annual flight hours, flight length of service and flight changes in the change of the gradient is small, the difference was not significant (P> 0.05). Under comparable sex and age, MNF (6.12 ± 0.42 ‰) and MNCF (5.50 ± 0.40 ‰) were slightly higher than those of non-smoking flight staff MNF (5.119 ± 0. 5 0 ‰) and MNCF (4.48 ± 0.46 ‰), but the difference was not significant (P> 0.05). Conclusion Cosmic radiation causes chromosome damage of civil aviation pilots. Conventional micronucleus culture has low sensitivity and can not reflect the difference of flight load. Smoking did not significantly increase flight crew chromosomal damage