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RNA干扰(RNA interference,RNA i)首先由Fire等[1]提出,为双链RNA(double strand RNA,dsRNA)介导的细胞内序列特异性转录后基因沉默过程,是生物体在进化过程中,抵御病毒感染及由于重复序列和突变引起基因组不稳定性的保护机制。RNA i技术现已广泛应用于逆基因分析和基因治疗等
RNA interference (RNAi) was first proposed by Fire et al [1] and is a double strand RNA (dsRNA) -mediated intracellular sequence-specific post-transcriptional gene silencing process, which is the process by which organisms evolve , Protection against viral infections and protection mechanisms that cause genomic instability due to repetitive sequences and mutations. RNAi technology is now widely used in reverse gene analysis and gene therapy