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目的:通过高脂饮食建立大鼠非酒精性脂肪性肝炎肝纤维化模型。 方法:以普通饲料+100g/kg猪油+20g/kg胆固醇组成的高脂饲料喂养SD大鼠,并设普通饲料喂养大鼠作为对照,在实验4,8,12,16,24wk分批处死。测定血脂和血清转氨酶,并通过HE染色和VG苦味酸染色观察肝脏肝细胞脂肪变性、炎症和纤维化程度。 结果:2组大鼠体重均呈进行性增长,模型组大鼠4wk起肝指数(肝脏湿质量/体质量)明显升高,血清TCh水平8wk起明显升高,12～24wk血清ALT明显升高。模型组4wk大鼠出现肝细胞脂肪变性,8wk表现为单纯性脂肪肝,12-24wk进展为脂肪性肝炎,且24wk所有造模大鼠均出现窦周纤维化,1例出现桥接纤维化。 结论:持续24wk的高脂饮食就可建立大鼠非酒精性脂肪性肝炎肝纤维化模型,该方法简便实用,成功率高。
OBJECTIVE: To establish a non-alcoholic steatohepatitis model of hepatic fibrosis in rats by a high-fat diet. Methods: SD rats were fed with high-fat diet consisting of normal diet + 100g / kg lard + 20g / kg cholesterol and normal rats were fed as control. The rats were sacrificed at 4, 8, 12, 16, 24wk . Blood lipids and serum aminotransferases were measured, and hepatic steatosis, inflammation and fibrosis were observed by HE staining and VG picric acid staining. Results: The body weight of rats in both groups increased progressively. The index of liver (wet weight / body weight of liver) in model group increased significantly from 4wk, serum TCh level increased significantly from 8wk, ALT increased significantly at 12 ~ 24wk . In the model group, the steatosis of hepatic cells appeared in 4wk rats, simple fatty liver appeared in 8wk, steatohepatitis was developed in 12-24wk, sinus fibrosis was found in all rats of 24wk, and bridge fibrosis in 1 patient. CONCLUSION: A non-alcoholic steatohepatitis model of liver fibrosis in rats can be established by a 24-week high-fat diet. The method is simple and practical with high success rate.