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目的 :研究Survivin在大肠癌中的表达及其与大肠癌增殖活性的相关意义。方法 :应用免疫组化S P法检测 64例大肠癌组织及 1 0例癌旁组织的Survivin、增殖细胞核抗原 (PCNA)蛋白表达水平。结果 :癌旁大肠黏膜组织中均无Survivin表达 ,Survivin在大肠癌的表达阳性率为 53 % ,PCNA的增殖指数为 33 .65± 1 8.7%。Survivin蛋白表达与大肠癌的组织学类型、淋巴结转移相关 (P <0 .0 5 ,P <0 .0 5) ,而与浸润深度无关 (P >0 .0 5) ;Survivin表达与PCNA的阳性表达呈正相关。Survivin阳性组的PCNA的增殖指数为 53 .8± 1 8.6 % ,明显高于Survivin阴性组的 1 3 .5± 3 .5 % (P <0 .0 5)。结论 :Survivin在大肠癌中高表达 ,在大肠癌的发生、发展中起着重要的作用 ,与大肠癌的分化程度、淋巴结转移、高增殖有关。
Objective: To investigate the expression of Survivin in colorectal carcinoma and its correlation with the proliferative activity of colorectal cancer. Methods: The expressions of Survivin and proliferating cell nuclear antigen (PCNA) protein in 64 cases of colorectal carcinoma and 10 cases of paracancerous tissues were detected by immunohistochemical SP method. Results: No expression of Survivin was found in adjacent colorectal mucosa. The positive rate of Survivin in colorectal carcinoma was 53% and the proliferative index of PCNA was 33.6 ± 8.7%. Survivin protein expression was correlated with histological type and lymph node metastasis (P <0.05, P <0.05), but not with depth of invasion (P> 0.05). Survivin expression was positively correlated with PCNA The expression was positively correlated. Proliferation index of PCNA in Survivin positive group was 53.8 ± 1.86%, which was significantly higher than that in Survivin negative group (P <0.05). Conclusion: Survivin is overexpressed in colorectal cancer and plays an important role in the occurrence and development of colorectal cancer, which is related to the differentiation of colorectal cancer, lymph node metastasis and hyperplasia.