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以NIH3T3细胞作为载体细胞，用基因转染的方法建立了持续分泌白细胞介素2（IL－2）的细胞株。在体外此细胞上清可以增强小鼠脾细胞和腹腔巨噬细胞对黑色素瘤B16细胞的杀伤作用（与对照组比较，均为P＜0．01）。体内注射基因转染细胞则可以抑制黑色素瘤细胞在体内的生长（与对照组比较，均为P＜0．01）。结果表明，分泌IL-2的成纤维细胞可以通过激活非特异性免疫杀伤细胞而达到抗肿瘤的作用，是一种有较大潜力的肿瘤生物治疗的新途径。
Using NIH3T3 cells as a vector, a cell line that secretes interleukin 2 (IL-2) was established by gene transfection. This cell supernatant in vitro can enhance the killing effect of mouse splenocytes and peritoneal macrophages on melanoma B16 cells (P<0.01 compared with the control group). Injection of the gene-transfected cells in vivo can inhibit the growth of melanoma cells in vivo (P<0.01 compared with the control group). The results showed that IL-2-secreting fibroblasts can achieve anti-tumor effects by activating non-specific immune killer cells, which is a new approach for biological treatment of tumors with great potential.