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目的:研究漏芦能否逆转胃癌相关成纤维细胞的促癌作用。方法:漏芦以0.32 g/kg、0.65 g/kg、1.3 g/kg、2.6 g/kg、5.2 g/kg的剂量灌服小鼠,制备含漏芦含药血清。胃癌相关成纤维细胞分别进行常规培养和含漏芦血清培养,制备条件培养液,培养AGS胃癌细胞。用MTT实验、划痕实验和Transwell实验检测AGS细胞增殖、迁移和侵袭能力的变化,用ELISA检测各组胃癌相关成纤维细胞培养液中TGFβ1和IL6含量的变化,以荧光定量PCR检测胃癌相关成纤维细胞中TGFβ1和IL6表达量的变化。结果:常规培养的胃癌相关成纤维细胞显著促进了AGS细胞的增殖、迁移和侵袭;1.3 g/kg、2.6 g/kg和5.2 g/kg组含漏芦血清培养胃癌相关成纤维细胞,使其促AGS细胞增殖、迁移和侵袭的能力明显降低,使其培养液中TGFβ1和IL6的含量明显下降,使其细胞内TGFβ1和IL6 mRNA的表达量明显下调,2.6 g/kg和5.2 g/kg组的效果尤为明显。结论:漏芦能抑制胃癌相关成纤维细胞对TGFβ1和IL6的分泌而逆转其促癌作用。
Objective: To investigate whether the leakage of Lou can reverse the carcinogenesis of gastric cancer-associated fibroblasts. Methods: The mice were fed with 0.32 g / kg, 0.65 g / kg, 1.3 g / kg, 2.6 g / kg and 5.2 g / kg respectively. The gastric cancer-associated fibroblasts were cultured in the conventional manner and the culture medium containing the lentinus edulis, respectively, and the conditioned medium was prepared to culture AGS gastric cancer cells. The changes of proliferation, migration and invasion of AGS cells were detected by MTT assay, scratch assay and Transwell assay. The changes of TGFβ1 and IL6 in gastric cancer-associated fibroblasts were detected by ELISA. Changes of TGFβ1 and IL6 expression in fibroblasts. RESULTS: Conventional cultured gastric cancer-associated fibroblasts significantly enhanced the proliferation, migration and invasion of AGS cells. Gastric cancer-associated fibroblasts were cultured in 1.3 g / kg, 2.6 g / kg and 5.2 g / kg groups, The ability of AGS cells to proliferate, migrate and invade was significantly decreased, and the levels of TGFβ1 and IL6 in the culture medium were significantly decreased. The expression of TGFβ1 and IL6 mRNA was significantly down-regulated in the medium of 2.6 g / kg and 5.2 g / kg The effect is particularly obvious. Conclusion: Lentinula can inhibit the secretion of TGFβ1 and IL6 in gastric cancer-associated fibroblasts and reverse their carcinogenic effect.