硒对大鼠急性缺血心肌Ca~(2+)-ATP酶活性的影响

• 作 者: 郑海燕 刘铸 张万年
• 机 构: 西安医科大学病理生理教研室,西安医科大学病理生理教研室,西安医科大学病理生理教研室
• 出 处: 中国病理生理杂志
• 发表日期: 1991年02期
• 关键词: 心肌 大鼠 心室游离壁 心肌细胞线粒体 右心室游离壁 缺血区 肌原纤维 左冠状动脉 下开 急性缺血

选用体重230～250gSD大鼠,随机分为缺血组(n=10)和加硒缺血组(n=10)。于全麻下开胸,结扎左冠状动脉,分别于结扎30min、60min迅速取下左心室游离壁缺血区,右心室游离壁非缺血区,按Fujimoto等方法进行Ca~(2+)-ATP酶细胞化学染色,用透射电镜观察,并计算各组心肌细胞线粒体大小(以μ计)。结果表明,正常心肌细胞Ca~(2+)-ATP酶活性产物存在于质膜、线粒体、肌浆网及肌原纤维。心肌缺血30min,与非缺

The weight of 230 ~ 250gSD rats were randomly divided into ischemia group (n = 10) and selenium plus ischemia group (n = 10). Thoracotomy was performed under general anesthesia. Left coronary artery was ligated. Left ventricular free wall and left ventricular free wall were rapidly removed at 30min and 60min after ligation, respectively. According to Fujimoto et al. Method, Ca ~ (2 +) - ATPase cytochemical staining, transmission electron microscopy, and calculate the mitochondrial size of myocardial cells in each group (in μ dollars). The results showed that the normal myocardial cells Ca ~ (2 +) - ATPase activity of the product exists in the plasma membrane, mitochondria, sarcoplasmic reticulum and myofibrils. Myocardial ischemia 30min, and not missing