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酵母多糖(Zymosa)是酵母菌细胞壁的成份。在37℃,有适量Mg~(++)存在时,酵母多糖能与C3结合形成酵母多糖-C3复合物(Z-C3),用Z-C3免疫动物可获得C3免疫血清,现介绍制备酵母多糖的方法,并以自制酵母多糖及进口酵母多糖与C3的复合物分别制备免疫血清,比较两种复合物的免疫原性及免疫效果。 材料与方法 一、酵母多糖的制备: 1.新鲜酵母500克,加0.5 M Na:HPO_42000 ml,在沸水浴中煮2～3小时,并不断搅拌,以溶介可溶性
Zymosa is a component of the yeast cell wall. Zymosan can combine with C3 to form zymosan-C3 complex (Z-C3) in the presence of a suitable amount of Mg ~ (++) at 37 ° C. C3 immune sera can be obtained by immunizing animals with Z-C3. Polysaccharides, and the immune sera were prepared from the homemade yeast polysaccharides and the complexes of zymosan and C3. The immunogenicity and immune effect of the two complexes were compared. Materials and methods First, the preparation of yeast polysaccharides: 1. 500 grams of fresh yeast, add 0.5 M Na: HPO_42000 ml, boiled in a boiling water bath for 2 to 3 hours, and continue stirring to dissolve soluble