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目的探讨24 h快速眼动睡眠剥夺(REMSD)对青少年期C57BL/6J小鼠空间学习记忆能力和海马一氧化氮(NO)水平的影响。方法 2014年1月—2015年1月选取45只青少年期(3~4周龄)SPF级雄性C57BL/6J小鼠,采用随机数字表法将小鼠分为对照组、宽平台组和REMSD组,各15只。将REMSD组小鼠置于水平台睡眠剥夺箱建立REMSD模型,宽平台组小鼠置于假水平台睡眠剥夺箱建立快速眼动睡眠假剥夺模型。通过3轮Morris水迷宫定位航行实验测试小鼠学习能力,记录逃避潜伏期;Morris水迷宫空间搜索实验测试小鼠记忆能力,记录小鼠穿台次数,在靶象限停留时间以及在靶象限游动距离。颈椎脱臼处死小鼠,分离海马,采用NO检测试剂盒检测NO水平。结果REMSD组小鼠出现多动,毛发潮湿凌乱且光泽度差,四肢充血,烦躁不安,易激惹,兴奋性增强。各组小鼠3轮Morris水迷宫定位航行实验逃避潜伏期比较,差异均有统计学意义(P<0.05);其中,REMSD组逃避潜伏期均较对照组、宽平台组缩短(P<0.05)。各组小鼠穿台次数比较,差异有统计学意义(P<0.05);其中,REMSD组小鼠穿台次数多于对照组和宽平台组(P<0.05)。对照组、宽平台组、REMSD组海马NO水平分别为(43.6±8.5)、(45.8±9.1)、(54.2±5.8)μmol/L,差异有统计学意义(F=7.460,P=0.002);其中,REMSD组小鼠海马NO水平高于对照组和宽平台组(P<0.05)。结论青少年期小鼠REMSD 24 h后,学习记忆能力提高,可能与海马NO水平升高有关。
Objective To investigate the effects of REM sleep on spatial learning and memory and nitric oxide (NO) levels in adolescent C57BL / 6J mice. Methods From January 2014 to January 2015, 45 male (3 to 4 weeks old) SPF male C57BL / 6J mice were selected and randomly divided into control group, wide platform group and REMSD group , Each 15. REMSD mice were placed in water platform sleep deprivation box to establish REMSD model, wide platform group mice were placed in a false water platform sleep deprivation box to establish rapid eye movement sleep deprivation model. Morris water maze spatial search experiment was used to test the memory ability of mice, the number of mice permutation was recorded, the dwell time in the target quadrant and the distance in the target quadrant were measured by three rounds of Morris water maze positioning navigation experiments. . The mice were sacrificed by cervical dislocation, the hippocampus was separated, and NO level was detected by NO detection kit. Results The mice in REMSD group showed hyperactivity, the hair was wet and messy, the gloss was poor, the extremities were hyperemia, restlessness, irritability and excitability. The escape latency of three groups of mice in the Morris water maze positioning navigation experiment were significantly different (P <0.05), and the escape latency in the REMSD group was shorter than that in the control group and the wide platform group (P <0.05). There were significant differences in the number of wearing trains in each group (P <0.05). Among them, the number of wearing trains in REMSD group was more than that in control group and wide platform group (P <0.05). The levels of NO in the hippocampus of the control group, the wide platform group and the REMSD group were (43.6 ± 8.5), (45.8 ± 9.1) and (54.2 ± 5.8) μmol / L, respectively, with statistical significance (F = 7.460, P = 0.002) Among them, NO level in hippocampus of REMSD group was higher than that in control group and wide platform group (P <0.05). Conclusion Adolescents with REMSD can improve the ability of learning and memory after 24 h, which may be related to the increase of NO in hippocampus.