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以四季秋海棠红铜叶系列和绿叶系列的叶片为外植体,对不定芽的诱导及试管苗的增殖进行了研究,结果表明:两种系列四季秋海棠叶片均不适合用75%乙醇消毒,适宜的消毒方法为0.1%升汞消毒7 min;在添加6-BA3.0 mg/L和NAA0.1mg/L的MS培养基上可分化大量不定芽,是不定芽诱导和试管苗增殖的较佳培养基。
In this study, adventitious bud induction and proliferation of in vitro seedlings were studied using the leaves of the four seasons Begonia brassicae series and the green leaf series as explants. The results showed that both series of Begonia septempunctata L. were not suitable for disinfection with 75% ethanol, Was disinfected with 0.1% mercuric chloride for 7 min. Adventitious shoots could differentiate in a large number of adventitious buds on MS medium supplemented with 6-BA3.0 mg / L and NAA0.1 mg / L, which was better for adventitious bud induction and plantlet proliferation Medium.