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目的:评估在原有血液检测技术的基础上增加核酸检测技术的效果。方法:献血者标本使用生化仪检测ALT,使用2种ELISA试剂检测HBsAg、抗-HCV抗体、抗-HIV抗体、抗-TP抗体,检测结果阴性及单试剂检测有反应性的标本进行核酸检测,核酸检测使用罗氏Cobas s201核酸检测系统。结果:核酸检测前37 296人份标本的传染病标志物阳性率为1.04%,核酸检测23 834人份,检出阳性标本35例,其中34例为HBV DNA阳性,1例为HIV RNA阳性,阳性率0.15%。结论:ELISA检测阴性血液存在较高的HBV感染风险,增加核酸检测技术可以进一步增强血液安全。
Objective: To evaluate the effect of adding nucleic acid detection technology on the basis of the original blood detection technology. Methods: The biochemical analyzer was used to detect ALT in blood donors. Two kinds of ELISA reagents were used to detect HBsAg, anti-HCV antibody, anti-HIV antibody and anti-TP antibody. Negative results and single reagents were used to detect the nucleic acid. Nucleic acid detection using Roche Cobas s201 nucleic acid detection system. Results: The positive rate of infectious disease markers in 37 296 samples before the nucleic acid test was 1.04% and 23 834 were detected in nucleic acid samples. 35 positive samples were detected, of which 34 were HBV DNA positive, 1 was HIV RNA positive, The positive rate of 0.15%. Conclusion: There is a high risk of HBV infection in negative blood samples by ELISA. Increasing nucleic acid detection can further enhance blood safety.