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探索气虚血瘀证结肠腺癌移植瘤的小鼠复合模型的建立及中药活血化瘀法干预对其影响。方法:健康8周龄Balb/c雌性小鼠72只随机分成6组,分别为:空白对照组(A组)、单纯荷瘤对照组(B组)、复合模型对照组(C组)、复合模型低剂量组(D组)、复合模型中剂量组(E组)、复合模型高剂量组(F组)。造模第16天起,A、B、C组给予生理盐水灌胃,D、E、F组分别按高、中、低剂量的四君子合血府逐瘀汤灌胃,每天1次连续4周。对造模前后小鼠进行症状和体征的评价,第42天,将已禁食12 h的各组小鼠称重、麻醉,检测局部微循环血流量,眼球取血以送检血液流变学。结果:1各复合模型组小鼠量化评分积分评价气虚血瘀程度皆为重度,但高剂量组活血化瘀药干预后较复合对照组量化积分明显减轻,差异有统计学意义(P<0.05)。2全血黏度按空白对照组、单纯荷瘤组、复合模型组呈梯度递增,运用中药干预后,呈现不同程度的下降。3空白组的局部血流量大于荷瘤组及复合模型组所测得血流值,其中耳缘血管(P<0.01)腹壁静脉(P<0.05)差异最为显著。复合模型组随活血化瘀药剂量的增加局部微循环血流量有逐步增加。4至造模第42天,单纯荷瘤组与复合模型组体质量均减轻,与空白组比较有极显著差异(P<0.01)。复合模型组体质量与单纯荷瘤组相比有减轻,随活血化瘀药剂量增加差异逐渐减小。结论:1本实验建立了气虚血瘀状态下Balb/c小鼠结肠腺癌复合模型,符合中医证型特点。2对气虚血瘀证结肠腺癌移植瘤的小鼠复合模型分别采用低、中、高剂量的活血化瘀中药进行干预,能不同程度减少气虚血瘀的症状与体征。
Establishment of a mouse model of colon adenocarcinoma xenografts with Qi - deficiency and Blood Stasis Syndrome and the effect of the intervention of promoting blood circulation to remove blood stasis on it. Methods: Seventy-two healthy 8-week-old Balb / c female mice were randomly divided into 6 groups: blank control group (A group), simple tumor-bearing control group (B group), complex model control group (C group) Low dose group (group D), middle dose group (group E) and high dose group (group F). Groups A, B and C were given normal saline intragastrically on the 16th day after model establishment. Groups D, E and F were intragastrically administered with high, medium and low doses of Sijunzi Xuefuzhuyu Decoction once daily for 4 weeks . The symptoms and signs of the mice before and after modeling were evaluated. On the 42nd day, the mice of each group that had been fasted for 12 hours were weighed, anesthetized, the blood flow of the local microcirculation was detected, and the eyeball blood was taken for examination of hemorheology . Results: 1 The quantification score points of each compound model group were all evaluated as severe, but the quantification scores of the high-dose group were significantly lower than those of the compound control group after intervention (P <0.05) . 2 whole blood viscosity according to the blank control group, simple tumor-bearing group, compound model group showed a gradient increase, the use of Chinese medicine intervention, showing varying degrees of decline. The blood flow in the blank group was greater than that in the tumor-bearing group and the compound model group, with the most significant difference in the veins (P <0.01). Compound model group with the increase of the dose of local blood circulation microcirculation blood flow increased gradually. From the 4th day to the 42nd day of modeling, the body weight of the simple tumor-bearing group and the compound model group were all reduced, with a significant difference compared with the blank group (P <0.01). Compared with the simple tumor-bearing group, the body weight of the compound model group was reduced, and the difference with the increase of the dose of the traditional Chinese medicine for promoting blood circulation and removing stasis was gradually reduced. Conclusions: 1 This experiment established Balb / c mouse colon adenocarcinoma complex model with Qi deficiency and blood stasis, in line with the characteristics of traditional Chinese medicine syndromes. 2 The compound model of mouse adenocarcinoma xenografts with Qi deficiency and blood stasis syndrome were treated with low, middle and high doses of traditional Chinese medicine of promoting blood circulation and removing blood stasis to reduce the symptoms and signs of qi deficiency and blood stasis in varying degrees.