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目的 发展一种快速准确的鉴定鼠疫菌的方法。方法 Ｐ Ｃ Ｒ 反应加限制性内切酶消化的方法。结果 对来自不同疫源地的 １０３ 株鼠疫菌 １６ Ｓ２３ Ｓｒ Ｒ Ｎ Ａ 基因间区进行扩增，均可扩出两条长为 １ １４６ｂｐ 及 １ ０９０ｂｐ 的片段，扩增产物用限制性内切酶 Ｔａｑ Ｉ， Ｍ ｓｐ Ｉ消化后的酶切图谱相同。而对照菌株小肠结肠炎耶尔森氏菌，鼠伤寒沙门氏菌，大肠杆菌，痢疾杆菌，霍乱弧菌的扩增产物及酶切图谱与鼠疫菌均不相同。结论 鼠疫菌 １６ Ｓ２３ Ｓｒ Ｒ Ｎ Ａ 基因间区高度同源，基本为两种类型，长度分别为 １ １４６ｂｐ 及 １ ０９０ｂｐ，其他菌株与其明显不同；该方法将有助于快速准确的鉴定鼠疫菌。
Objective To develop a rapid and accurate method for the identification of Yersinia pestis. Methods P C R reaction plus restriction endonuclease digestion method. Results The 16 S23 Sr R N A region of 103 Y. pestis isolates from different foci of origin were amplified and two fragments of 1 146 bp and 1 090 bp in length were amplified. Digested enzyme Taq I, M sp I after digestion digestion map the same. The control strains Yersinia enterocolitica, Salmonella typhimurium, Escherichia coli, Shigella, Vibrio cholerae amplification products and digestion maps and Y. pestis are not the same. Conclusion The 16 S23 Sr R N A region of Y. pestis is highly homologous and has two basic types, with lengths of 1 146 bp and 1 090 bp, respectively, which are obviously different from other strains. This method will be helpful for the rapid and accurate identification of plague bacteria.