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目的研究人宫颈上皮内瘤变和浸润癌组织中树突状细胞和T细胞的数量、分布变化,为人宫颈癌的诊断和生物治疗提供实验依据。方法采用免疫组织化学和图像分析技术,测定人宫颈上皮内瘤变、宫颈浸润癌和正常宫颈组织中树突状细胞表型抗原CD1a、S-100及T细胞表面分化抗原CD3、CD8阳性细胞的数量、分布和表达强度。结果与人正常宫颈组织比较,宫颈上皮内瘤变中CD1+a、S-100+、CD3+、CD8+细胞数量增多(P<0.05),与组织分级呈正相关(P<0.05),抗体表达强度增高;宫颈浸润癌中4种抗体阳性细胞数量减少,与临床分期呈负相关(P<0.05),强度略有下降。结论人宫颈上皮癌前病变至浸润癌变过程中树突状细胞和T细胞数量及抗体表达强度发生变化,提示宫颈病变局部的免疫功能发生了改变。
Objective To study the changes of the number and distribution of dendritic cells and T cells in human cervical intraepithelial neoplasia and invasive carcinoma and to provide experimental evidence for the diagnosis and biological treatment of human cervical cancer. Methods Immunohistochemistry and image analysis were used to determine the expression of dendritic cell surface antigen CD1a, S-100 and T cell surface differentiation antigen CD3, CD8 positive cells in human cervical intraepithelial neoplasia, invasive cervical carcinoma and normal cervical tissue Quantity, distribution and expression intensity. Results Compared with normal cervical tissue, the number of CD1 + a, S-100 +, CD3 + and CD8 + cells in cervical intraepithelial neoplasia increased (P <0.05), and positively correlated with histological grade (P <0.05) ; The number of 4 kinds of antibody-positive cells in invasive cervical cancer decreased, and the clinical stage was negatively correlated (P <0.05), the intensity decreased slightly. Conclusions The number of dendritic cells and T cells in human precancerous lesions of the cervix and the intensity of antibody expression change during the process of invasive carcinogenesis, suggesting that local immunologic function of cervical lesions has changed.