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目的探讨不同检测方法对于不规则抗体的检测效果。方法随机选取2013年1月—2015年2月期间接受红细胞不规则抗体筛查输血患者2448例,分别采用微柱凝胶法、聚凝胺法、抗球蛋白法、酶法、盐水法对其进行抗进行检测,对各种方法的检测效果进行对比分析。结果本次研究中,2448例患者的不规则抗体检测阳性率为0.49%;同木瓜酶法、凝聚胺法、抗人球蛋白法相比,微柱凝胶法对于IgG抗体的检测效价明显高于其他三种,其敏感性最佳。结论对于需要检测不规则抗体的标本,可先行盐水法检测排除Ig M抗体,再采用微柱凝胶法进行更为准确的IgG抗体筛选,从而有效避免迟发性溶血性输血反应。
Objective To investigate the detection of irregular antibodies by different detection methods. Methods A total of 2448 patients who received RBC irregular blood transfusions during January 2013 to February 2015 were enrolled in this study. They were treated with micro-column gel method, polybrene method, antiglobulin method, enzyme method and saline method respectively Conduct anti-detection, comparative analysis of the detection results of various methods. Results In this study, the positive rate of irregular antibody test in 2448 patients was 0.49%. Compared with papain enzymatic method, polybrene method and anti-human globulin method, the detection titer of IgG antibody was significantly higher The other three, the best sensitivity. Conclusion For samples that need to detect irregular antibodies, IgM antibody can be excluded by saline method first and then more accurate IgG antibody screening by microcolumn gel method, so as to avoid delayed hemolytic transfusion reaction.