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合成了一种新的L-天冬酰胺邻香草醛席夫碱和1,10-邻菲咯啉的氧钒配合物[VO(o-Van-L-Asn)(Phen)]1.5CH3OH(o-Van-L-Asn=邻香草醛与L-天冬酰胺形成的席夫碱,Phen=1,10-邻菲咯啉).利用红外光谱对其进行了表征,并通过X射线单晶衍射测定了其晶体结构.该晶体属于三斜晶系,P-1空间群,晶胞参数为:a=0.98990(10)nm,b=1.21591(11)nm,c=1.28349(12)nm,α=66.8180(10)°,β=83.816(2)o,γ=66.4150(10)o,V=1.2992(2)nm3,Dc=1.430 g cm-3,Z=1,F(000)=580,R1=0.0626,wR2=0.1631.通过紫外吸收光谱、荧光光谱、圆二色光谱(CD)和粘度测定等方法研究了该配合物与小牛胸腺DNA(CT-DNA)的相互作用.结果表明,配合物以插入方式与CT-DNA结合.光谱法研究该配合物与牛血清白蛋白(BSA)的相互作用表明,它主要以静态猝灭方式使BSA的内源性荧光发生猝灭,可引起蛋白构象的变化.计算得到了其结合常数Kb和结合位点数n.
A new kind of vanadyl complex of VO-o-Van-L-Asn (Phen)] 1.5CH3OH (o -Van-L-Asn = Schiff base formed from o-vanillin and L-asparagine, Phen = 1,10-phenanthroline) .It was characterized by FTIR and characterized by X-ray single crystal diffraction The crystal structure of the crystal belongs to the triclinic, P-1 space group with a = 0.98990 (10) nm, b = 1.21591 (11) nm and c = 1.28349 = 66.8180 (10) °, β = 83.816 (2) o, γ = 66.4150 (10) o, V = 1.2992 (2) nm3, Dc = 1.430 g cm-3, R1 = 0.0626, wR2 = 0.1631.The interaction between the complex and calf thymus DNA (CT-DNA) was studied by ultraviolet absorption spectroscopy, fluorescence spectroscopy, circular dichroism spectroscopy (CD) The complex was inserted into the CT-DNA.It was found that the interaction of the complex with bovine serum albumin (BSA) by spectroscopy indicated that it mainly quenches the endogenous fluorescence of BSA by static quenching, Conformational changes of the protein were calculated by its binding constant Kb and the number of binding sites n.