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在植物组织培养中,再生植株有两条途径:一是胚胎发生型(胚状体);二是器官发生型。目前水稻胚胎发生型的研究和应用正引起人们的关注。与器官发生型相比,胚胎发生植株再生率高,遗传变异小,可作为细胞工程中快速繁殖的重要手段。另外,人工种子的生产和原生质体培养也必须通过这一途径。为研究胚胎发生的途径,势必对胚性愈伤组织和非胚性愈伤组织进行早期识别。本试验采用解剖镜和扫描电镜观察、摄影等实验手段对这两类愈伤组织作了外部形态上的比较,同时用细胞学方法比较胚性细胞和非胚性细胞之间内部超微结构上的差异,并用生化测定的方法进行了蛋白电泳分析。为愈伤组织的形态发生提供了一些理论依据。
In plant tissue culture, regenerated plants have two pathways: one is embryogenic (embryoid body); the other is organogenesis. At present, the research and application of rice embryogenesis is arousing people’s attention. Compared with the organogenesis type, embryo plant regeneration rate is high, genetic variation is small, can be used as an important means of rapid propagation in cell engineering. In addition, the production of artificial seeds and protoplast culture must also pass this route. In order to study the pathways of embryogenesis, embryonic callus and non-embryogenic callus must be identified early. In this experiment, anatomical and scanning electron microscope observations, photographs and other experimental means for these two types of callus made an external morphological comparison, at the same time by cytological methods compared embryonic cells and non-embryonic cells between the internal ultrastructure The difference and biochemical determination of protein electrophoresis. It provided some theoretical basis for the morphogenesis of callus.