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采用成蚊药帐接触法和击倒试验法，对湖北省溴氰菊酯浸帐灭蚊实施六年后媒介按蚊对药物是否产生抗药性进行了现场调查。结果显示：１、媒介按蚊接触１５ｍｇ／ｍ２溴氰菊酯药帐３ｍｉｎ的死亡率均＞９０％，其敏感性没有显著下降，尚未产生抗性。说明接触小剂量溴氰菊酯，媒介按蚊产生抗药性的速度是较为缓慢的。２、农业上使用拟除虫菊酯较多的仙桃市，媒介按蚊接触浸药帐纱的ＫＴ５０与农业上使用拟除虫菊酯较少的恩施市有非常显著的差异（ｔ＝２６．６７，Ｐ＜０．０１），与浸帐地区有显著差异（Ｆ＝３．６８，Ｐ＜０．０５）。表明农业上日益广泛地使用拟除虫菊酯可能导致媒介按蚊产生抗药性。
Using mosquito drug tentative contact method and knockdown test method, a field investigation was conducted on whether the mosquito was resistant to the drug or not after six years of mosquitoes dipped in deltamethrin in Hubei Province. The results showed that: 1, the mortality of Anopheles mosquitoes exposed to 15 mg / m2 deltamethrin for 3 minutes was> 90%, their sensitivity did not decrease significantly and no resistance was found. This indicates that the exposure of small dose of deltamethrin to mosquitoes is relatively slow. 2. In Xiantao City with more pyrethroid in agriculture, KT50 of Anopheles stephens exposed to drenched yarn was significantly different from that of Enshi City with less pyrethroid in agriculture (t = 26.67, P < 0.01), which was significantly different from that of the dipped area (F = 3.68, P <0.05). Suggesting that the increasingly widespread use of pyrethroids in agriculture may lead to resistance to Anopheles vectors.