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High-resolution two-dimensional gel electropho-resis (2-DE), followed by computer-assisted image analysis was used to screen protein pattes of normal and infarcted myocardial tissues for quantitative and qualitative differencesin protein expression. In the gels of pH 5-8 immobilizedpH gradient (IPG) strips, 851 protein spots were detected in normal myocardial tissue and 1 032 protein spots were resolved in infarcted myocardial tissue. Thirteen protein spots only expressed in normal myocardial tissue, and 14 protein spots only expressed in infarcted myocardial tissue. Results also showed that 49 protein spots displayed quantitative changes in expression between normal and infarcted myocar-dial tissue. Eleven protein spots were subjected to mass spectrometry (MS) analysis and seven proteins were identi-fied by peptide mass fingerprinting (PMF). These proteins may be involved in cardiovascular injury, and could play an important role in the treatment of coronary heart disease.