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目的 :观察急性心肌梗死 (AMI)后左室梗死区 (LVIZ)糖蛋白 1 3 0 (GP1 3 0 )表达的动态变化与左室重构(LVRM)的关系 ,以及氯沙坦对GP1 3 0表达的影响。方法 :AMI术后 2 4h存活的 73只雄性Wistar大鼠随机分为 :AMI组及氯沙坦组 ,AMI组又依照术后 1 ,3 ,7,1 4,2 1 ,2 8d分为 6组 ,氯沙坦组于术后第 2天起灌胃给药 ,连续 4周。另设假手术组及正常组各 8只为对照。行心脏标本病理分析 ,分别用放射免疫法和氯氨T法测定LVIZ血管紧张素Ⅱ (AngⅡ )和羟脯氨酸(HC)含量 ,用免疫组化法检测LVIZGP1 3 0蛋白水平的表达。结果 :AMI后 ,心脏质量 (HW)、左室质量 (LVW)、左室质量指数 (LVWI)、HC均显著增加 (P <0 .0 5或P <0 .0 1 ) ;AMI组LVIZAngⅡ明显升高 ,与LVRM有相关性 (均P <0 .0 1 ) ;LVIZGP1 3 0蛋白表达在AMI第 1天开始即明显增加 ,7d达高峰 ,以后有所下降 ,但2 8d时仍明显高于假手术组 (P <0 .0 1 ) ;相关分析显示LVIZGP1 3 0蛋白表达与LVIZAngⅡ、LVRM参数间呈正相关(P <0 .0 5或P <0 .0 1 ) ;与AMI2 8d组相比 ,氯沙坦组LVRM明显减轻 ,GP1 3 0蛋白表达明显下调 (P <0 .0 5或P<0 .0 1 )。结论 :大鼠AMI后LVIZGP1 3 0基因的过度表达与LVRM的发生密切相关 ,氯沙坦改善LVRM的机制还可能与抑制GP1 3 0基因的过度
OBJECTIVE: To observe the relationship between the dynamic changes of glycoprotein 130 (GP1 3 0) and left ventricular remodeling (LVRM) in acute myocardial infarction (AMI) and the effect of losartan on GP1 3 0 The impact of expression. METHODS: Seventy-three male Wistar rats surviving 24 hours after AMI were randomly divided into AMI group and losartan group. AMI group was divided into 6 groups according to 1, 3, 7, 14, 21 and 28 days after operation Group, losartan group on the first 2 days after administration of gavage for 4 weeks. Another set of sham-operated group and normal group of 8 as a control. Pathological analysis of cardiac samples was performed. The contents of LVII, AngⅡ and hydroxyproline (HC) were measured by radioimmunoassay and chloramine T method, respectively. The expression of LVIZGP1 30 protein was detected by immunohistochemistry. Results: After AMI, the cardiac mass (HW), left ventricular mass (LVW), left ventricular mass index (LVWI) and HC increased significantly (P <0.05 or P <0.01) (All P <0.01). LVIZGP1 30 protein expression increased significantly on the first day of AMI, peaked on the 7th day, then decreased, but remained significantly higher on the 28th day than that of the LVRM (P <0.01). Correlation analysis showed that LVIZGP1 30 protein expression was positively correlated with LVIZAngⅡand LVRM parameters (P <0.05 or P <0.01). Compared with AMI2 8d group, , Losartan group LVRM significantly reduced, GP1 3 0 protein expression was significantly reduced (P <0.05 or P <0.01). CONCLUSION: The overexpression of LVIZGP1 30 gene after AMI is closely related to the occurrence of LVRM. The mechanism of Losartan in improving LVRM may be related to the suppression of GP130 gene over-expression