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目的:研究银杏提取物对氧化低密度脂蛋白诱导血管内皮细胞损伤后的影响及其可能的机制。方法:通过200μg/mL氧化低密度脂蛋白损伤血管内皮细胞模型,考察银杏提取物经氧化低密度脂蛋白损伤后血管内皮细胞生长方面的影响,并采用Western blot方法探索其可能的机制。结果:银杏提取物在5～40μg/mL的浓度能抑制氧化低密度脂蛋白对血管内皮细胞的损伤,其机制可能是通过下调BAX蛋白的表达及上调Bcl-2蛋白的表达途径抑制氧化低密度脂蛋白对血管内皮细胞的损伤。结论:银杏提取物能通过调控凋亡蛋白的途径有效抑制氧化低密度脂蛋白对血管内皮细胞的损伤。
Objective: To investigate the effect of Ginkgo biloba extract on oxidative low density lipoprotein-induced vascular endothelial cell injury and its possible mechanism. Methods: The vascular endothelial cell model was induced by 200μg / mL oxidized low density lipoprotein (LDL) to investigate the effects of Ginkgo biloba extract on the growth of vascular endothelial cells after oxidative low density lipoprotein (LDL) injury. The possible mechanism was explored by Western blot. Results: Ginkgo biloba extract inhibited the injury of vascular endothelial cells by low density lipoprotein (LDL) at the concentration of 5 ~ 40μg / mL by down-regulating the expression of BAX protein and up-regulating the expression of Bcl-2 protein, Lipoprotein Damage to Vascular Endothelial Cells. Conclusion: Ginkgo biloba extract can effectively inhibit the damage of vascular endothelial cells by oxidized low density lipoprotein through the regulation of apoptotic proteins.