论文部分内容阅读
目的观察中药平颤方加褪黑素(MT)对N-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)所致帕金森病(PD)模型大鼠行为学和前脑多巴胺(DA)及其代谢产物含量的影响,探讨中药与MT联合治疗PD的疗效及作用机制。方法SD大鼠60只,分5个组:对照组、模型组、MT组、中药组、中药+MT组。模型组和各治疗组大鼠腹腔注射MPTP建立PD模型,用爬杆法和迷宫实验测量大鼠行为学改变和智力状况,用高压液相电化学法测定前脑DA、高香草酸(HVA)及二羟基苯乙酸(DOPAC)含量。结果PD模型大鼠出现不同程度爬杆能力下降和智力减退,与模型组相比各治疗组大鼠行为学异常可部分改善(P<0.01);模型组DA、HVA、DOPAC含量均较对照组显著降低(P<0.01),各治疗组DA、HVA、DOPAC含量较对照组低,但较模型组明显增高(P<0.05,P<0.01)。结论平颤方与MT联合通过调节大鼠前脑DA神经元活性从而改善PD症状。
Objective To observe the behavior of rat model of Parkinson’s disease (PD) induced by N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in rats with Pingqiao Fang plus melatonin (MT) To study the effects of dopamine (DA) and its metabolites on learning and forebrain, to investigate the therapeutic effect and mechanism of combination of Chinese medicine and MT on PD. Methods Sixty SD rats were divided into 5 groups: control group, model group, MT group, traditional Chinese medicine group, traditional Chinese medicine + MT group. PD model was established by intraperitoneal injection of MPTP in model group and each treatment group. Behavioral changes and mental status of rats were measured by climbing pole method and labyrinth test. Forebrain DA and homovanillic acid (HVA) were measured by high-pressure liquid electrochemical method. And dihydroxyphenylacetic acid (DOPAC) content. RESULTS: PD model rats exhibited different degrees of decline in climbing ability and mental decline. Compared with the model group, the behavioral abnormalities of the rats in the treatment groups were partially improved (P<0.01); the levels of DA, HVA and DOPAC in the model group were higher than those in the control group. Significantly decreased (P <0.01), DA, HVA, DOPAC levels in the treatment groups were lower than the control group, but significantly higher than the model group (P <0.05, P <0.01). Conclusion Pingzhi Fang combined with MT can improve the PD symptoms by regulating the activity of DA neurons in the forebrain of rats.