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目的 :用化学发光法测定生物制剂样品中SOD活性并消除样品中天然自由基清除剂黄酮类及维生素C、E等成分的干扰。方法 :分别用黄嘌呤———黄嘌呤氧化酶及邻苯三酚自氧化作为酶和非酶超氧阴离子自由基发生体系 ,以鲁米诺为发光剂 ,通过样品对发光的抑制率测定其SOD活性 ;并通过加热样品使SOD失活测得样品发光抑制率本底值 ,扣除以消除干扰。结果 :用本法消除干扰后 ,同一样品在两种体系中测得结果有高度一致性 ,SOD活性分别为 170U/ml和 16 5U/ml;不经加热处理直接测定值为 42 6 5U/ml和 2 75U/ml。结论 :本法用于测定生物制剂中SOD活性 ,可方便有效地消除黄酮类及维生素C、E等成分的干扰
OBJECTIVE: To determine the activity of SOD in biological samples and eliminate the interference of natural free radical scavenger flavonoids, vitamins C, E and other components by chemiluminescence. Methods: Xanthine-xanthine oxidase and pyrogallol autoxidation were used as free radical generating system of enzyme and non-enzymatic superoxide anion respectively. Luminol was used as luminant to determine its luminescence inhibition rate SOD activity; and by heating the sample to inactivate SOD measured sample luminescence inhibition rate background value deducted to eliminate interference. Results: After the interference was eliminated by this method, the results of the same sample in the two systems were highly consistent and the SOD activities were 170U / ml and 165U / ml, respectively. The direct determination value without heat treatment was 42 6 5U / ml And 2 75 U / ml. Conclusion: This method is used to determine the activity of SOD in biological agents, which can easily and effectively eliminate the interference of flavonoids and vitamin C, E and other components