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对同宗配合疫霉种——恶疫霉(PhytophthoracactorumSchroter)抗甲霜灵突变株和抗地茂散突变株的抗药性在游动孢子和卵孢子后代的遗传分析,筛选到抗性分别由细胞核纯合显性单基因控制的抗甲霜灵突变株和由细胞质可稳定遗传的线粒体基因控制的抗地茂散突变株。将携带抗甲霜灵对地茂散敏感(即MtrCns)标记的亲本与携带抗地茂散对甲霜灵敏感(即MtsCnr)标记的亲本共同培养,在200个有性生殖单卵孢后代中检测到携带3种不同抗药性标记的单孢株,其中32株携带MtrCns标记,165株携带MtsCnr标记,另有3株同时携带双亲的标记性状即MtrCnr。结果表明:恶疫霉种内不同菌株共同培养时,有性生殖以配对亲本的各自自交为主,同时配对亲本间也可发生一定频率的杂交。
The genetic analysis of the resistance of the isolates to Phytophthoracactorum Schroter and Methanosporium mutants was carried out in the progenies of zoospores and oospores, Metaxalone-resistant anti-metalaxyl mutant and an anti-maomai mutant controlled by the mitochondrial gene that can be stably inherited in cytoplasm. The parents carrying methimazole-susceptible Mtrix (MtrCns) were cocultured with parents carrying metalaxyl-sensitive (MtsCnr) -labeled anti-metalaxyl, Monosirulent strains carrying three different drug resistance markers were detected. Among them, 32 strains carried MtrCns marker, 165 strains carried MtsCnr marker and 3 strains also carried parental marker MtrCnr. The results showed that when P. coelicolor was co-cultivated with different strains, sexual reproduction was mainly paired with their own parents, and a certain frequency of crosses could also occur between mating parents.