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目的了解胶体金免疫层析法在鼠疫监测中的作用及其优劣。同时观察56℃30 min灭活与升汞盐水作用1 min对鼠疫菌F1抗体检测的影响。方法对2006年从广西隆林县、西林县鼠疫监测中收集的鼠脏器、鼠血清以及既往鼠疫抗体阳性患者血清,利用胶体金免疫层析技术进行鼠疫菌F1抗原(脏器)与F1抗体(血清)检测。结果鼠肝脏120份、鼠脾脏99份,胶体金法检测鼠疫菌Fl抗原均为阴性;鼠血清235份胶体金法检测鼠疫菌F1抗体为阴性、既往鼠疫阳性病人27例,胶体金检测鼠疫菌Fl抗体灭活组阳性2例,阳性率为7.41%(2/27),升汞组均为阴性。结论升汞对鼠疫菌F1抗体的检测有抑制作用;胶体金免疫层析法具有特异性强、敏感性高、操作简便、快速等优点,在鼠疫监测中具有重要意义。
Objective To understand the role of colloidal gold immunochromatography in the surveillance of plague and its advantages and disadvantages. At the same time, the effect of inactivation of 30 min at 56 ℃ and mercuric salt water for 1 min on the detection of Yersinia pestis F1 antibody were observed. Methods The serum of murine and mouse sera collected from the plague surveillance in Longlin and Xilin counties of Guangxi in 2006 and the sera of patients with positive past plague antibodies were used to detect the relationship between the F1 antigen (organ) of Yersinia pestis and F1 antibody (Serum) test. Results 120 mice liver and 99 spleen mice were detected by colloidal gold method. The results of colloidal gold assay showed that all the antibodies against Y. pestis F1 were negative, Fl antibody inactivation group was positive in 2 cases, the positive rate was 7.41% (2/27), mercury group were negative. Conclusions Mercury has an inhibitory effect on the detection of Y. pestis F1 antibody. Colloidal gold immunochromatography has the advantages of high specificity, high sensitivity, simple and rapid operation, and is of great significance in the surveillance of plague.