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本研究以海陆渐渗系13-1×辽棉12组配的195个单株的F2群体为作图群体,构建遗传连锁图谱,挖掘与纤维品质相关稳定的QTL,为标记辅助13-1选择育种提供依据。本研究利用SSR标记和Joinmap3.0软件构建遗传连锁图谱,并通过Ici Mapping完备区间作图法对F2及F2:3家系进行纤维品质性状QTL定位。结果显示:构建的遗传连锁图谱包含39个多态性标记、13个连锁群,该图谱总长1 174.4 c M,覆盖棉花基因组的26.7%;共检测到37个与纤维品质相关的QTL,其中伸长率2个,整齐度9个,马克隆值19个,比强度7个,分布在10个染色体上。19个有利等位基因来自海陆渐渗系13-1,18个有利等位基因来自辽棉12,发现两个稳定的QTL位点,可作为海陆渐渗系棉花纤维品质基因功能研究的候选基因。本研究筛选出的多态性标记可辅助前期选择。
In this study, F2 populations from 195 individuals of 13-1 × Liaolian 12 combinations were used as mapping population to construct genetic linkage map and to find QTLs related to fiber quality. Breeding to provide the basis. In this study, SSR markers and Joinmap3.0 software were used to construct genetic linkage map, and QTL mapping of F2 and F2: 3 lines was performed by Ici Mapping complete interval mapping. The results showed that the genetic linkage map consisted of 39 polymorphic markers and 13 linkage groups. The total length of the linkage map was 1 174.4 cM, covering 26.7% of the total genome of cotton. A total of 37 QTLs related to fiber quality were detected, Length 2, uniformity 9, micronaire 19, specific intensity 7, distributed in 10 chromosomes. Nineteen favorable alleles were from Hailuanyin osmotic line 13-1 and 18 favorable alleles were from Liaodian12. Two stable QTLs were found, which could be used as candidate genes for studying the function of cotton fiber quality genes . Polymorphic markers screened in this study can be used to aid in the early selection.