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目的采用小鼠骨髓细胞微核试验和精子畸形试验研究铁皮石斛对小鼠的致突变作用。方法将50只昆明种小鼠随机分为5组。以不同剂量(5000 mg/kg、2500 mg/kg、1250 mg/kg)的铁皮石斛分2次经口灌胃染毒小鼠,每次间隔24 h,于末次染毒后6 h颈椎脱臼法处死小鼠,取股骨骨髓常规制片,Giemsa染色,在油镜下检测骨髓嗜多染红细胞微核率;另将25只雄性昆明种小鼠按上述剂量和分组经口灌胃连续染毒小鼠5 d,于首次染毒后第35 d用颈椎脱臼法将小鼠处死,取其双侧附睾制成精子悬液后制片,伊红染色,在高倍镜下观察精子畸形率。两项实验同时设阴性对照组(纯水)和阳性对照组(环磷酰胺40 mg/kg)。结果铁皮石斛各剂量组均未见致小鼠骨髓嗜多染红细胞微核和精子畸形作用。结论在本实验条件下,铁皮石斛对小鼠无致突变作用。
Objective To study the mutagenic effect of Dendrobium candidum on mice by using the micronucleus test of bone marrow cells and sperm abnormality test. Methods Fifty Kunming mice were randomly divided into five groups. Mice were inoculated with Dendrobium officinale with different dosages (5000 mg / kg, 2500 mg / kg and 1250 mg / kg) by oral gavage twice a day for 24 h. After cervical dislocation The mice were sacrificed and the bone marrow of femur was routinely prepared and stained with Giemsa to detect the micronuclei rate of bone marrow polychromatic erythrocytes under an oil microscope. Another 25 male Kunming mice were continuously inoculated by oral administration of the above doses and groups The mice were sacrificed on the 35th day after the first exposure and were sacrificed by cervical dislocation. The spermatozoa were used to prepare the epididymis and the eosin staining was performed. The sperm deformity rate was observed under high magnification. Both experiments set negative control (pure water) and positive control group (cyclophosphamide 40 mg / kg). Results All doses of Dendrobium candidum did not induce micronuclei and sperm deformity in mouse bone marrow polychromatic erythrocytes. Conclusion In this experiment, Dendrobium candidum had no effect on mice.