论文部分内容阅读
目的 研究结直肠癌组织趋化因子 IL - 8的表达与结直肠癌生物学行为的关系。 方法 采用 RT-PCR方法检测临床收集的新鲜结直肠癌组织标本中趋化因子 IL - 8m RNA的表达 ;采用免疫组织化学方法检测结直肠癌组织中趋化因子 IL - 8蛋白的表达。 结果 12例结直肠癌组织经 RT- PCR检测均可出现趋化因子 IL - 8m RNA的表达。 4 0例结直肠癌组织 IL - 8蛋白表达的阳性率为 87.5 % ;结直肠癌组织 IL - 8蛋白的表达与结直肠癌的转移及 Dukes分期有关 ,表达强者 ,转移发生率高 ,Dukes分期晚。 结论 结直肠癌组织中趋化因子 IL - 8的表达与结直肠癌的生物学行为有关
Objective To investigate the relationship between the expression of chemokine IL-8 in colorectal cancer and the biological behavior of colorectal cancer. Methods The expression of chemokine IL-8 mRNA in freshly collected colorectal cancer tissue samples was detected by RT-PCR. The expression of chemokine IL-8 protein in colorectal cancer tissue was detected by immunohistochemistry. Results Expression of chemokine IL-8 mRNA could be detected in 12 colorectal cancer tissues by RT-PCR. The positive rate of the expression of IL-8 protein in colorectal cancer tissue was 87.5 %. The expression of IL-8 protein in colorectal cancer tissue was related to the metastasis and Dukes stage of colorectal cancer. Strong expression was found in the colorectal cancer tissue. The incidence of metastasis was high. Dukes Late in phases. Conclusion The expression of chemokine IL-8 in colorectal cancer is related to the biological behavior of colorectal cancer