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目的：研究Ｆａｓ和可溶性Ｆａｓ 在急性淋巴细胞白血病（ＡＬＬ）中的表达，探讨Ｆａｓ在白血病细胞凋亡障碍中的作用。方法：分别用免疫组化ＳＡＢＣ法和双抗夹心ＥＬＩＳＡ 法，检测３６ 例ＡＬＬ患者和１０ 例正常对照组骨髓细胞Ｆａｓ 抗原（ｍ Ｆａｓ）表达以及血浆可溶性Ｆａｓ（ｓＦａｓ）的水平。用半定量逆转录－聚合酶链式反应（ＲＴ－ＰＣＲ）检测１２ 例ＡＬＬ患者和７ 例正常对照骨髓细胞Ｆａｓｍ ＲＮＡ 的表达。结果：３６ 例ＡＬＬ初诊组患者Ｆａｓ 抗原的表达较正常对照组低〔（６．２３±６．５８）％ｖｓ （２８．６３±５．０２）％ ， Ｐ ＜ ０．００１〕，血浆中ｓＦａｓ 水平也较正常对照组低〔（１１．８ １７６±４．７ ６２０）ｎｇ／ｍ ｌｖｓ （２７．２ ８７４±９．２ ７７８） ｎｇ／ｍ ｌ， Ｐ＜ ０．００１〕。ＡＬＬ组未见Ｆａｓ ｍ ＲＮＡ经转移剪接后缺乏跨膜区的１ １０４ ｂｐ（对应于ｓＦａｓ）条带，而正常对照组可见；ＡＬＬ组Ｆａｓ ｍ ＲＮＡ的表达较正常对照组也显著降低（０．３ ４０７ ｖｓ ０．８ ３０３， Ｐ＜ ０．００１）。结论：Ｆａｓ 可能参与了白血病细胞的凋亡障碍，ＡＬＬ白血病细胞可能不是通过提高血浆ｓＦａｓ 水平来逃避凋亡。白血病细?
Objective: To investigate the expression of Fas and soluble Fas in acute lymphoblastic leukemia (ALL) and to explore the role of Fas in the apoptosis of leukemic cells. Methods: The expression of Fas antigen (m Fas) and the level of soluble Fas (sFas) in 36 ALL patients and 10 normal controls were detected by immunohistochemical SABC and double antibody sandwich ELISA respectively. The expression of Fas mRNA in 12 ALL patients and 7 normal control bone marrow cells was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: The expression of Fas antigen in 36 cases of newly diagnosed ALL patients was lower than that of the control group [(6.23 ± 6.58)% vs (28.63 ± 5.02)%, P <0.001) Levels were also lower than in the normal control group (11.8 176 ± 4.7 620 ng / m lvs (27.2 874 ± 9.2 778) ng / m l, P <0.001). In ALL group, there was no 1 104 bp (corresponding to sFas) band of Fas m RNA after transfer splicing but not in normal control group. The expression of Fas m RNA in ALL group was significantly lower than that in normal control group (0. 3 407 vs 0.8 303, P <0.001). Conclusion: Fas may be involved in the apoptosis of leukemic cells. ALL leukemia cells may not escape apoptosis by increasing plasma sFas level. Leukemia thin?